![SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (% SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%](https://cdn.numerade.com/ask_previews/6f0c47ee-38d7-4778-b4d8-d117b7d1affe_large.jpg)
SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
![SOLVED: A rudimentary method to calculate the melting temperature of DNA is an equation known as "The Wallace Rule." This equation is: Tm = 2(A + T) + 4(G + C) Where SOLVED: A rudimentary method to calculate the melting temperature of DNA is an equation known as "The Wallace Rule." This equation is: Tm = 2(A + T) + 4(G + C) Where](https://cdn.numerade.com/ask_images/e6d23eaac0be47f7af19c3bc2e728e3d.jpg)
SOLVED: A rudimentary method to calculate the melting temperature of DNA is an equation known as "The Wallace Rule." This equation is: Tm = 2(A + T) + 4(G + C) Where
![Nucleic Acid Design Applications Polymerase Chain Reaction (PCR) Calculating Melting Temperature (Tm) PCR Primers Design. - ppt download Nucleic Acid Design Applications Polymerase Chain Reaction (PCR) Calculating Melting Temperature (Tm) PCR Primers Design. - ppt download](https://images.slideplayer.com/18/5707607/slides/slide_13.jpg)